BioPhase Empower Project Files – Revision History
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1.3 Revision A – 19 Jul 2023
- Initial Release
- Total Methods Included:
  - CE-SDS: 10 methods
  - cIEF: 8 methods
  - Fast Glycan: 8 methods
  - RNA 9000: 8 methods
  - Service: 21 methods

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1.3 Revision B – 09 Nov 2023
- Service Project Folder Updates:
  - Renamed “Service” project folder to “Sciex Service”
  - Removed UV and LIF BioPhase PQ sample set
  - Removed UV BioPhase PQ sample set
  - Removed Test Mix B PQ report
  - Updated Noise and Drift instrument method to run for 30 minutes at 2 Hz due to Empower software limitation
  - Updated LIF signal-to-noise instrument method to pressure separation to align with BioPhase methods
  - Renamed “Shutdown Air Dry” instrument methods to “Shutdown”
  - Updated custom field “DriftStatus” from 0.03 to 0.009 AU/hour
  - Updated custom field “NoiseStatus” from 0.000025 AU to 0.000035 AU to compensate for running noise and drift at 2 Hz
- Total Methods Included:
  - CE-SDS: 10 methods
  - cIEF: 8 methods
  - Fast Glycan: 8 methods
  - RNA 9000: 8 methods
  - Service: 18 methods

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1.4 Revision A – 09 May 2024
- Added DNA 20kb Project Folder
- CE-SDS Project Folder:
  - Added new methods:
    - CESDS Shutdown for Storage
    - Lightning Non-Reduced CESDS Sep
    - Lightning Reduced CESDS Sep
- RNA 9000 Project Folder:
  - Renamed methods for clarity
  - Added new method: RNA 9000 Separation Press Inj
- Sciex Service Project Folder:
  - Added Rapid Conditioning instrument method and method set
  - Updated Noise and Drift processing method to calculate noise from 5 min to 30 min
  - Updated LIF processing method to remove peak constant criteria for signal-to-noise
  - Updated “LIFStatus” custom field to include signal-to-noise criteria
- Total Methods Included:
  - CE-SDS: 16 methods
  - cIEF: 8 methods
  - DNA 20kb: 18 methods
  - Fast Glycan: 8 methods
  - RNA 9000: 10 methods
  - Service: 20 methods

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1.4 Revision B – 04 Apr 2025
- Enabled full audit trail for all project folders
- Sciex Service Project Folder Updates:
  - Added custom fields to convert:
    - AU → µAU
    - AU/hour → µAU/min
  - Updated UV Noise and Drift report method to display µAU for noise and µAU/min for drift
  - Added LIF Crosstalk instrument method, method set, and sample set
  - Added UV Crosstalk instrument method, method set, and sample set
  - Corrected UV Drift specification from 0.009 AU/hour to 0.09 AU/hour or 150 µAU/min
  - Updated Rapid Capillary Conditioning instrument method
  - Updated UV Noise and Drift report to include new custom field result
- Total Methods Included:
  - CE-SDS: 16 methods
  - cIEF: 8 methods
  - DNA 20kb: 18 methods
  - Fast Glycan: 8 methods
  - RNA 9000: 10 methods
  - Service: 26 methods

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1.5 Revision A – 26 Aug 2025
- CESDS Project Folder:
  - Added NFD related methods
- cIEF Project Folder:
  - Added NFD related methods
- Sciex Service Project Folder Updates:
  - Added NF Signal-to-Noise instrument method, method set, sample set, and processing methods
  - Added NF Crosstalk instrument method, method set, sample set, and processing methods
  - Added UV Crosstalk processing method
  - Added NF_LIF Crosstalk processing method
  - Added Crosstalk report
  - Added capillary conditioning sample set
  - Added LIF Signal-to-Noise sample set
  - Added NF Signal-to-Noise sample set
  - Added Rapid Capillary Conditioning sample set
  - Added Shutdown Air Dry sample set
  - Added System Performance LIF Only sample set
  - Added System Performance NF Only sample set
  - Added System Performance UV with NF sample set
  - Added UV Noise and Drift sample set
  - Added UV Test Mix B sample set
  - Updated LIF Crosstalk instrument method to run at 2 Hz, 4 sec peak width
  - Updated UV Crosstalk instrument method to run at 2 Hz, 4 sec peak width
  - Added custom field “NFStatus” for NF Signal-to-Noise
  - Added NF Signal-to-Noise report
  - Removed Rapid Conditioning and Shutdown Air Dry method sets from System Performance UV and UV with LIF sample sets
  - Updated all report methods:
    - Corrected cutoff fields
    - Added Sample Set ID to system info
    - Added Result ID, Date Acquired, and Date Processed to peak table
    - Updated header/footer to use Empower default info
  - Added versioning for traceability:
    - Initial methods: no version label
    - Updated methods: labeled V2, V3, V4, etc.
- Total Methods Included:
  - CE-SDS: 24 methods
  - cIEF: 14 methods
  - DNA 20kb: 18 methods
  - Fast Glycan: 8 methods
  - RNA 9000: 10 methods
  - Service: 46 methods

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1.6 Revision A – 26 Sep 2026
- Added Oligo 200 nt project folder with methods
- Enabled Full Audit Trail Settings in system policies and created new project folders
  - Set Project Objects: Methods, Results, Sample, Deletion to "Unrestricted"
  - Set Deletion to "Confirm Identity"
